SABOURAUD DEXTROSE AGAR (SDA) +Lec+Tw80+Hyst+S.Thio

APPLICATION

Sabouraud Dextrose Agar (SDA) is a non selective isolation medium used for the growth and maintenance of pathogenic and non-pathogenic fungi from clinical and non-clinical specimens. It is also used for recovery and total counting of yeasts and moulds in environmental monitoring.

This medium complies with the recommendations of the harmonized method in the United States Pharmacopoeia (USP) and European Pharmacopoeia (EP).

PRINCIPLE AND INTERPRETATION

The high concentration of dextrose and the acidic pH of the medium permit selectivity of fungi. The inactivation of residues of disinfectants is critical for the detection of viable and cultivable microorganisms in pharmaceutical production environments. For this purpose, different neutralizer combinations are added to the medium. Lecithin neutralizes quaternary ammonium compounds, Tween 80 is effective against phenolic compounds and mercurial derivates, Histidine inactivate aldehydes, Sodium thiosulfate neutralizes halogen compounds.